Welcome to the Medical and MolecularW Physiology Graduate ProgramW
at the
     

Institute for Biogenesis Research
Research Description

Allsopp

Richard Allsopp, Ph.d
Assistant Professor
Graduate Program Co-Chair

Phone:  (808) 692-1412
Fax:  (808) 692-1962
Email:  allsopp@hawaii.edu
Bioscience Building 163B, Kaka’ako

Ph.D. Pharmaceutical Chemistry, University of California at Berkeley, San Francisco, 1960Ph.D. Biochemistry, McMaster University, Canada 1996

Stem Cell Biology

Mechanisms Governing Survival and Telomerase Regulation in Stem Cells

Telomeres are essential genetic elements that cap the ends of chromosomes.  The replication of telomeres in proliferating cells is accomplished by a specialized ribonucleic acid enzymatic complex called telomerase.  I have been studying telomere shortening and cell aging as well as telomerase biology, especially regulation of telomerase, for over a decade.  More recently, as a post doctoral fellow in Dr. Irving Weissman’s lab at Stanford University, I became interested in stem cell biology, especially the regulation of telomerase in stem cells.  I joined the Institute for Biogenesis Research (IBR) in the summer of 2003 as an assistant professor, where I am at present. My current research interests at the IBR are still in the area of stem cell biology and telomere/telomerase biology, and also include research on factors controlling and effecting long-term stem cell survival.  I am particularly interested in figuring out how telomerase is regulated in stem cells, and what role telomerase dysregulation may have during perinatal development.  I am also interested in the role that a new gene, Sirt1, may play in different types of stem cells.  Sirt1 is an NAD-dependent protein deacetylase that effects both cell survival and has been implicated as a transcriptional regulator of telomerase as well.  Stem cells which I am particularly interested in include blood stem cells, germ line stem cells and neuronal stem cells.

Table 1: Differentially expressed genes .pdf

Selected Publications

  1. Allsopp, R. C., Vaziri, H., Patterson, C., Goldstein, S., Younglai, E. V., Futcher, A. B., Greider, C. W. and Harley, C. B. Telomere length predicts replicative capacity of human fibroblasts. 1992. Proc. Nat. Acad. Sci. U. S. A. 89, 10114-10118. .pdf

  2. Vaziri, H., Dragowska, W., Allsopp, R. C., Thomas, T. E., Harley, C. B. and Lansdo

rp, P. Evidence for a mitotic clock in primative blood stem cells: Loss of telomeric DNA with age. 1994. Proc. Nat. Acad. Sci. U.S.A.  91, 9857-9860. .pdf

  1. Greenberg, R., Allsopp, R ., Chin, L., Morin, G. and dePinho, R. Expression of mouse telomerase reverse transcriptase during development, differentiation and proliferation. 1998.  Oncogene, 16, 1723-30. .pdf
  2. Allsopp, R., Cheshier, S. and Weissman, I.L. Telomere shortening accompanies increasedcell cycle activity during serial transplantation of hematopoietic stem cells. 2001. J. Exp. Med. 193, 917-924. .pdf
  3. Allsopp, R., Cheshier, S. and Weissman, I.L. Telomerase activation and rejuvenation oftelomere length in activated of T cells derived from hematopoietic stem cells following serial   transplantation. 2002. J. Exp. Med. 196, 1427-33. .pdf
  4. Allsopp, R., Morin, G., DePinho, R., Harley, C. and Weissman, I.L. Telomerase is required to slow telomere shortening and extend replicative lifespan of HSC during serial transplantation.   2003. Blood. 102, 517-20. .pdf
  5. Allsopp, R., Morin, G., DePinho, R., Harley, C. and Weissman, I.L. Effect of TERT over-expression on the long-term transplantation capacity of hematopoietic stem cells. 2003. Nat. Med. 9, 369-71. .pdf
  6. Allsopp, R., Shimoda, J.,  Easa, D.,  and  Ward, K. Long Telomeres in the Mature Human    Placenta. Placenta. In Press. 2006. .pdf
  7. Coussens, M., Yamazaki, Y., Moisyadi, S., Suganuma, R., Yanigimachi, R. and Allsopp, R. Regulation and effects of modulation of telomerase reverse transcriptase expression inprimordial germ cells during development. 2006. Biol. Reprod. 75, 785-791. .pdf