Genomics Core Facilty Services

Currently we are offering services in the areas of qPCR, Microarray, Sequencing, and Other Genomic Services.

If you have recurring processes that you would like to automate or have us take over, we would be glad to sit down and talk with you to discuss how we can help you acheive your goals.

We are aiming to be a self-sustaining entity consisting solely on a chargeback system. Our prices reflect the costs of equipment service contracts, potential and actual maintenence costs, as well as direct and indirect labor costs and administration. Our costs are kept low by being able to provide services in bulk with high throughput, it is therefore sometimes necessary to have minimum orders or fees in order to maintain a positive cost structure.

qPCR Microarray Sequencing Bioanalyzer

qPCR

Absolute and relative quantitative gene expression using SYBR Green chemistry. Applied Biosystems 7900HT System using Power SYBR Green Chemistry. Supply cDNA template and primers and we will run the rest for you.
We also have a product that can perform micro RNA profiling using qPCR for very accurate and reproducible results.

Microarray

Gene expression and Comparative Genomic Hybridization assays using Agilent arrays and G2565CA Scanner. For Gene Expression, just provide us with Total RNA and we will take care of the rest.

Sequencing

96 well Applied Biosystem 3730xl Capillary Sequencer for reads up to 1000 bases. We will run the cycling reaction and cleanup for you and supply you with resulting sequence and quality of calls.

Other Genomic Services

Bioanalyzer RNA/DNA quality checks, robotic liquid handling, micro RNA profiling and microplate absorbance and fluorescence detection. We can offer various forms of quality checks for your samples, or create custom processing for your high throughput needs.

Background
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In March of 2009 the Cardiovascular and Ob/Gyn departments initiated a project to establish a Genomics Core Facility here at JABSOM to serve the needs of the campus, UH Manoa, and Hawaii. The scope of services offered may expand or contract depending on needs but we currently offer services in the areas of microarrays, real-time PCR, sequencing, as well as additional equipment and services as needed.

We would like as much feedback as possible in these initial stages to help establish the services that would be beneficial to the greatest number of researchers.
The supervisor for the core is Steffen Oeser. He worked for 8 years at Illumina, Inc and served in numerous roles including Lab Manager, Assay Developer, Product Developer, and Project Manager

  • Automation - We automate many of the steps in our process to generate reproducible, quality data.

  • Experience - The Genomics Core Director, Steffen Oeser, has over 7 years experience performing assay and product development in a high throughput Biotech company, Illumina, Inc.

  • Quality - We regularly service and maintain our core equipment. A majority of our equipment is under service contract to ensure that data is reliable and reproducible.

  • Protocols - We have detailed protocols for every step of every service. All experiments are performed exactly the same way, every time.

  • Speed - For even the most complex of services, data can be returned within the week. For some of our basic services, turnaround is within 24 hours.

  • Pricing - Currently our pricing is extremely affordable compared to other service providers. Keep in mind that our equipment and services are geared towards high-throughput processes. Optimal pricing is based on running a full plate of samples for such processes as qPCR and sequencing. Fewer than a full plate of samples will mean higher per-sample charges. Estimates for all pricing is calculated on the request forms so surprise charges are not possible. You will never pay more than what is quoted on a request form.

Newsletter
Highlights
  • Dec 23rd-Jan 2nd

    The Core will be closed for the holidays. Please do not send or submit any new samples until on or after Jan 3rd as no one will be available to accept them.

  • Nov 16, 2010

    If you have used, or currently use the Core for any services, please download and submit a survey to let us know how we are doing. We require input to provide the best quality service and justify our continued presence.

  • Jun 28, 2010

    Kyan Agbayani begins work as a new student hire for the Genomics Core Facility.

  • Jun 14, 2010

    Entire Genomics Core Facility is moved to 3rd floor of the BSB building (room 309).

  • May 24, 2010

    genomics@hawaii.edu becomes default e-mail address for contacting the Genomics Core Facility.

  • Nov 3, 2009

    1-2:15pm Jane Hutchinson from Roche 454 gives the following talk at JABSOM Campus: "Updates on 'Next Generation' Sequencing Technology and Applications" email if interested. BSB Room 320N.

  • Oct 20, 2009

    12:30-2pm Andrea Bayer from ABI gives the following talk at JABSOM Campus: "The basics of Real Time PCR, to assay development, or troubleshooting and MicroRNA solutions." email Core to RSVP, food provided. BSB Room 320N.

  • Mar 03, 2009

    Steffen Oeser comes to the University of Hawaii to start working on setting up the Genomics Core Facility at the University of Hawaii, JABSOM campus.

Contact Info
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Genomics Core Facility
University of Hawaii, JABSOM
651 Ilalo St.
Honolulu, HI 96813
Core Hours: 8:30a-4p
Service Hours: listed on request forms
Email: genomics@hawaii.edu

Phone: 808.692.1860
Fax: 808.692.1966
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