Applied Biosystems 3730xl DNA Analyzer 96-Well Capillary Sequencer

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DNA Sequencing Information :

The sequence of DNA encodes the necessary information for living things to survive and reproduce. Determining the sequence is therefore useful in fundamental research into why and how organisms live, as well as in applied subjects. Because of the key nature of DNA to living things, knowledge of DNA sequence may come in useful in practically any biological research. For example, in medicine it can be used to identify, diagnose and potentially develop treatments for genetic diseases. Similarly, research into pathogens may lead to treatments for contagious diseases. Biotechnology is a burgeoning discipline, with the potential for many useful products and services.

DNA sequencing is the process of determining the nucleotide order of a given DNA fragment. Thus far, most DNA sequencing has been performed using the chain termination method developed by Frederick Sanger. This technique uses sequence-specific termination of a DNA synthesis reaction using modified nucleotide substrates. However, new sequencing technologies such as Pyrosequencing are gaining an increasing share of the sequencing market.

Applied Biosystem utilizes the process of labeling the terminators, commonly called 'dye terminator sequencing'. The major advantage of this approach is the complete sequencing set can be performed in a single reaction, rather than the four needed with the labeled-primer approach. This is accomplished by labeling each of the dideoxynucleotide chain-terminators with a separate fluorescent dye, which fluoresces at a different wavelength.

Applied Biosystems

DNA Analyzer 3730xl
The 96-capillary 3730xl DNA Analyzer is the Gold Standard for high throughput genetic analysis. Use this for DNA fragment analysis applications such as microsatellites, AFLP, SNP analysis, mutation detection and traditional DNA sequencing. Get the highest quality data at a low cost per sample.
To Run Sequencing Samples Through The Genomics Core:

  • Step 1: Prepare template in any of the available formats listed in the Sequencing Request Form(PCR Products, Gel Isolated DNA Fragments, Plasmids, etc.)

  • Step 2: Pick up skirted PCR plate from Core if you do not have one available.

  • Step 4: Drop off your template in the supplied skirted PCR plate according to instructions in the Sequencing Request Form.

  • Step 5: The Core will e-mail you with the results containing: sequence and quality metrics for base calls.